Platelet-Leucocytes Aggregates (PLA) The new important test for identification of thombic events in RSAs and failed IVFs
Formation of platelet-leukocyte-aggregates (PLA) represents an important mechanism by which leukocytes contribute to thrombotic events and platelets to inflammatory reactions. Damage to a blood vessel results in the adhesion of platelets to the site of injury and the subsequent formation of a platelet thrombus. Interaction of leukocytes with activated platelets is coordinated by an adhesion cascade in which P-selectin binds to P-selectin glycoprotein ligand-1 (PSGL-1) on leukocytes.
Purpose and methodology
Increased platelet-leucocyte aggregate has been reported in women that had RSAs or failed IVFs because of the thrombotic events that were diagnosed afterwards. This test can help not only for diagnostic purposes, but also to guide the physicians in order to perform the appropriate therapy with low molecular heparin for successful women’s pregnancy. This test can be performed in combination with the genetic thrombophilia panel testing. The methodology used for this test is flow cytometry and a green top tube is needed to run the test.