Nowadays, a couple has to be very careful before getting the crucial decision to go into IVF procedure. The couple can go on IVF if the husband produces no sperm, and if woman has reproductive anatomical issues or has no good ovaries. Then they have to do some important testing.

First of all, husband has to be excluded that his sperm is ok, not fragmented. Then woman has to be excluded that has no problem his ovaries in her reproductive anatomical structured. She should be also checked that is not compatible with the husband (blocking factors test) and immunology testing such as NK activation, embryotoxicity and PLA.

If they regulate all these factors then they will have high chances to get successful IVF.

The quality of sperm is not determined ONLY by the regular sperm analysis that shows the motility and the number of sperms, but DNA fragmentation is a very precise biomarker and very crucial to be tested for every male that wants to have children.

This is because this marker scientifically has been proven to be important for infertility, RSAs, and failed IVFs. Also, the fault is the when they see that a sperm has high motility and adequate number of spermatozoa; they think that the male is ok, when they never check DNA fragmentation.

Checking the DNA fragmentation, women can be protected for spending money and time if sperm is the problem for their reproductive issues.

NK cell activation has been proven to be important in infertility, RSAs, and failed IVFs. NK factor can influence reproduction for about 60-70 percent.

NK number is a totally different test compared to NK activation test and most women have to be very careful for this because they may have a normal NK number but their activity is abnormal and this affects the success of a pregnancy of IVF.

However, when the number of NK cells is increased, then the NK activity may be normal or NOT. So, the very crucial impact for successful pregnancy is the NK activity test and not the NK numbers by itself.

The anti-parental antibodies is a very important issue for fertility because it has to do with the histocompatability (blocking factors) to wife and husband.

This issue in the past showed cases of couples with blocking factors that had RSAs or failed IVFs and the treatment was a vaccine for infusion of male’s lymphocytes to women, but this therapy was abolished by USA FDA.

If women choose to vaccinate they have to be careful because it has been proven that ONLY one vaccination is enough to restore the problem for the whole year. During this time, the couple has many chances to have their baby. Also, for women that choose not the vaccination, today there is infusion therapy that lasts for 6 months.